However, the environmental changes in routine cell culture are poorly understood even for the staple cell lines used in biomedical research. Cells have evolved sophisticated pathways to sense and respond to minute changes in the in vivo microenvironment 5, 6, 7, 8, 9 and presumably, the same mechanisms govern cellular behaviors in cell culture. Hence, as cells grow, the culture medium acidifies and deoxygenates to create conditions potentially inconsistent with the levels and stability of the in vivo environment 4, although the extent of these changes depends on numerous factors, including for example, cell density, the rate of cellular respiration, and the rate of diffusive flux at the air-liquid interface 1. However, cells growing in culture consume O 2 and release CO 2 through cellular metabolism. In contrast, pH levels in routine cell cultures are governed by the equilibrium between media formulations (with a finite buffering capacity) and the CO 2-enriched atmosphere (usually adjusted to 5 or 10% CO 2), whereas levels of dissolved gases are governed by surface/atmosphere equilibration. In the mammalian body, there are numerous regulatory systems (e.g., vasodilation, vasoconstriction, changes in respiration rates, and vascular remodeling 2, 3) that maintain levels of dissolved gases and acid-base chemistry within the finite limits that sustain optimal cell function. One of the most striking discrepancies between routine in vitro mammalian cell cultures and the in vivo environment is the lack of regulatory systems that finely tune levels of oxygenation and acid-base chemistry in the mammalian body to maintain homeostasis 1. The degree to which cell culture conditions mimic the in vivo environment and recapitulate the natural behavior and function of cells plays a critical role in the biological relevance of the observations made. These findings should catalyze further efforts to increase the relevance of experimental results to the in vivo physiology and enhance reproducibility.
Our results show that in vitro cell cultures consistently undergo large departures of environmental parameters during standard batch culture. We collate data from the literature to demonstrate that standard cell cultures consistently exhibit environmental instability, indicating that this may be a pervasive issue affecting experimental findings. Here, we perform frequent in situ monitoring of dissolved O 2 and CO 2 with optical sensor spots and contemporaneous evaluation of cell proliferation and medium pH in standard batch cultures of three widely used human somatic and pluripotent stem cell lines. This has led to a focus on the influence of cell culture conditions on cellular responses and reproducibility of experimental findings. I own two of these and use one in the coach and one in the wet bay.Mammalian cell cultures are a keystone resource in biomedical research, but the results of published experiments often suffer from reproducibility challenges. I contacted Ideal Sciences and they have a newer version which I tested and it works outstanding, problem solved, and they did this as a courtesy proving their outstanding customer service.
ZER TEMP MONITOR CELLULAR OFFLINE
When I first got mine I had a problem where when the Internet went offline for whatever reason the temp stick would not reconnect and the battery would die and it was useless.
ZER TEMP MONITOR CELLULAR HOW TO
(most text messaging systems have an email address that can be used to send texts, just google how to to do for your carrier).
ZER TEMP MONITOR CELLULAR FREE
Monitoring is free which is built in to the up front cost of $149 for the unit, there is a web portal and smart phone app you can enjoy with the ability to easily create alerts which can send you text messages, etc. I highly recommend the Temp Stick by Ideal Sciences which I chose years ago. The other scenario is I use these for winter time ops for basement temperature monitoring. Summer is approaching and RVers with pets often want a system that can alert them when temps get above a threshold such as if shore power goes out while away and the air conditioners are not cooling.
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